The Las Vegas PRP Hair Treatment is
ideal for all those patients who are suffering from androgenic alopecia for at
least six months and are on finasteride or topical minoxidil without much of an
improvement. There is a written consent required from the patient after which
the necessary blood tests are conducted to find out the platelet count and
other diseases if any including HIV. Patients suffering from thyroid
dysfunction, hematological disorders, malnutrition or other dermatological
disorders are exempted from such treatments. A one square centimeter area is
marked over the parietal area on the mid-pupillary line, and the baseline
follicular units are counted manually with the help of a trichoscan
machine.
Before the start of the session, hair pull test is also
conducted in the same area at least three times and washing of hair is
prohibited for at least two days before the treatment. The hair pull test in
conducted by grasping a bunch of hair between the thumb, index and middle
finger and the hair extracted An anesthetic in each session. linear in the
evaluation of the overall hair volume and growth, quality, and fullness of the
hair, and pictures ccs from all different angles. This is necessary for the
treatment procedure, and this hair pulls test is also necessary for the
preparation of the PRP.
After such evaluation the preparation of the PRP is done by
collecting at least 20 cc of fresh blood. This collection of blood is done with
proper aseptic precautions taken in a minor operation theater and the blood is
collected in a vacutainer containing sodium citrate solution. These tubes are
then rotated in the centrifugal machine which rotates at a speed of 15oo
revolutions each minute for six minutes altogether. The soft spin of the first
centrifugation is done to separate the blood into three different layers according
to the plasma content.
The layers get arranged accordingly with the topmost layer is
the cellular plasma layer containing about forty percent of the plasma and is
thereby called the platelet poor plasma or PPP. The PRP layer in the middle is
called the Buffy Coat and contains around five percent of the total volume. The
layer at the bottom is called the RBC layer which contains the rest of the
total volume. The Buffy Coat is separated further in a test tube with the help
of a Finn pipette and is sent for another round of centrifugation called hard
spin as it is faster at 1500 RPM and done for a longer period of 15 minutes.
The
PRP settles at the bottom, and the upper layer of PPP is discarded. 9 part of
this PRP is now collected in the insulin syringe which contains one part of
calcium chloride which would become the activator. An anesthetic cream is
applied over the bald area, cleaned with and antiseptic, and the PRP is
injected using Nappage technique in which multiple injections are made each 1 cm
apart in a linear pattern. With 2 to 3 cc injected every week for four weeks,
you can see the result for yourself.
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